bafilomycin a1 Search Results


bafilomycin a  (InvivoGen)
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bafilomycin a1  (Tocris)
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bafilomycin a1  (Santa Cruz Biotechnology)
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bafilomycin a1  (TargetMol)
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bafilomycin a1 s1413  (Selleck Chemicals)
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bafilomycin a1  (Valiant Co Ltd)
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bafilomycin a1  (MedChemExpress)
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bafilomycin a1  (Biosynth Carbosynth)
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PINK1-53 expression is enhanced in the presence of Lys-63-linked ubiquitination. A, anti-PINK1 immunoblots of lysates prepared from control or PINK1-transfected HEK293 cells that were either untreated or treated with 1 μm MG132, 1 μm PSI, 5 μm Lactacytsin, 10 mm 3-methyladenine (3MA), and 200 nm <t>Bafilomycin</t> <t>A1</t> for 16 h or 10 μm CCCP for 4 h, as indicated. The bands corresponding to full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) are indicated. The blots above were stripped and reprobed with anti-actin as a loading control. This experiment was repeated at least three times. B, representative immunoblots of at least three experimental sets showing the expression level of full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) in the presence of 1 μm MG132 (16 h) or various forms of HA-ubiquitin (Ub). The numbers indicate the average -fold (Avg. fold) change in the densitometric level of PINK1-53. C, same as B, except that HA-ubiquitin was replaced by myc-Ubc13, myc-UbcH7, FLAG-parkin, or HA-HHARI. The asterisk denotes cells treated with 1 μm MG132. This experiment was duplicated. D, representative immunoblots of at least three experimental sets showing the expression level of full-length (63 kDa) and PINK1-53 (53 kDa) in the presence of Ubc13 coexpression alone or with GFP-Ataxin-3, HA-UCHL-1, HA-SENP8, or GFP. (DUB, Deubiquitinating enzyme). E, same as C, except that PINK1 is expressed in some cases in the presence of both myc-Ubc13 and FLAG-parkin or HA-HHARI. The blots above were stripped and reprobed with anti-actin antibody to reflect loading variations. This experiment was duplicated. F, the relative chymotrypsin-like proteasome activities of at least three sets of lysates prepared from PINK1 overexpressing cells cotransfected with various E2s and E3s as indicated. **, p < 0.001 compared with the first column; Student's t test.
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chemicals bafilomycin a1 baf  (LKT Laboratories)
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LKT Laboratories chemicals bafilomycin a1 baf
PINK1-53 expression is enhanced in the presence of Lys-63-linked ubiquitination. A, anti-PINK1 immunoblots of lysates prepared from control or PINK1-transfected HEK293 cells that were either untreated or treated with 1 μm MG132, 1 μm PSI, 5 μm Lactacytsin, 10 mm 3-methyladenine (3MA), and 200 nm <t>Bafilomycin</t> <t>A1</t> for 16 h or 10 μm CCCP for 4 h, as indicated. The bands corresponding to full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) are indicated. The blots above were stripped and reprobed with anti-actin as a loading control. This experiment was repeated at least three times. B, representative immunoblots of at least three experimental sets showing the expression level of full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) in the presence of 1 μm MG132 (16 h) or various forms of HA-ubiquitin (Ub). The numbers indicate the average -fold (Avg. fold) change in the densitometric level of PINK1-53. C, same as B, except that HA-ubiquitin was replaced by myc-Ubc13, myc-UbcH7, FLAG-parkin, or HA-HHARI. The asterisk denotes cells treated with 1 μm MG132. This experiment was duplicated. D, representative immunoblots of at least three experimental sets showing the expression level of full-length (63 kDa) and PINK1-53 (53 kDa) in the presence of Ubc13 coexpression alone or with GFP-Ataxin-3, HA-UCHL-1, HA-SENP8, or GFP. (DUB, Deubiquitinating enzyme). E, same as C, except that PINK1 is expressed in some cases in the presence of both myc-Ubc13 and FLAG-parkin or HA-HHARI. The blots above were stripped and reprobed with anti-actin antibody to reflect loading variations. This experiment was duplicated. F, the relative chymotrypsin-like proteasome activities of at least three sets of lysates prepared from PINK1 overexpressing cells cotransfected with various E2s and E3s as indicated. **, p < 0.001 compared with the first column; Student's t test.
Chemicals Bafilomycin A1 Baf, supplied by LKT Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bafa1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc bafa1
PINK1-53 expression is enhanced in the presence of Lys-63-linked ubiquitination. A, anti-PINK1 immunoblots of lysates prepared from control or PINK1-transfected HEK293 cells that were either untreated or treated with 1 μm MG132, 1 μm PSI, 5 μm Lactacytsin, 10 mm 3-methyladenine (3MA), and 200 nm <t>Bafilomycin</t> <t>A1</t> for 16 h or 10 μm CCCP for 4 h, as indicated. The bands corresponding to full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) are indicated. The blots above were stripped and reprobed with anti-actin as a loading control. This experiment was repeated at least three times. B, representative immunoblots of at least three experimental sets showing the expression level of full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) in the presence of 1 μm MG132 (16 h) or various forms of HA-ubiquitin (Ub). The numbers indicate the average -fold (Avg. fold) change in the densitometric level of PINK1-53. C, same as B, except that HA-ubiquitin was replaced by myc-Ubc13, myc-UbcH7, FLAG-parkin, or HA-HHARI. The asterisk denotes cells treated with 1 μm MG132. This experiment was duplicated. D, representative immunoblots of at least three experimental sets showing the expression level of full-length (63 kDa) and PINK1-53 (53 kDa) in the presence of Ubc13 coexpression alone or with GFP-Ataxin-3, HA-UCHL-1, HA-SENP8, or GFP. (DUB, Deubiquitinating enzyme). E, same as C, except that PINK1 is expressed in some cases in the presence of both myc-Ubc13 and FLAG-parkin or HA-HHARI. The blots above were stripped and reprobed with anti-actin antibody to reflect loading variations. This experiment was duplicated. F, the relative chymotrypsin-like proteasome activities of at least three sets of lysates prepared from PINK1 overexpressing cells cotransfected with various E2s and E3s as indicated. **, p < 0.001 compared with the first column; Student's t test.
Bafa1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bafilomycin a1  (BOC Sciences)
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BOC Sciences bafilomycin a1
PINK1-53 expression is enhanced in the presence of Lys-63-linked ubiquitination. A, anti-PINK1 immunoblots of lysates prepared from control or PINK1-transfected HEK293 cells that were either untreated or treated with 1 μm MG132, 1 μm PSI, 5 μm Lactacytsin, 10 mm 3-methyladenine (3MA), and 200 nm <t>Bafilomycin</t> <t>A1</t> for 16 h or 10 μm CCCP for 4 h, as indicated. The bands corresponding to full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) are indicated. The blots above were stripped and reprobed with anti-actin as a loading control. This experiment was repeated at least three times. B, representative immunoblots of at least three experimental sets showing the expression level of full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) in the presence of 1 μm MG132 (16 h) or various forms of HA-ubiquitin (Ub). The numbers indicate the average -fold (Avg. fold) change in the densitometric level of PINK1-53. C, same as B, except that HA-ubiquitin was replaced by myc-Ubc13, myc-UbcH7, FLAG-parkin, or HA-HHARI. The asterisk denotes cells treated with 1 μm MG132. This experiment was duplicated. D, representative immunoblots of at least three experimental sets showing the expression level of full-length (63 kDa) and PINK1-53 (53 kDa) in the presence of Ubc13 coexpression alone or with GFP-Ataxin-3, HA-UCHL-1, HA-SENP8, or GFP. (DUB, Deubiquitinating enzyme). E, same as C, except that PINK1 is expressed in some cases in the presence of both myc-Ubc13 and FLAG-parkin or HA-HHARI. The blots above were stripped and reprobed with anti-actin antibody to reflect loading variations. This experiment was duplicated. F, the relative chymotrypsin-like proteasome activities of at least three sets of lysates prepared from PINK1 overexpressing cells cotransfected with various E2s and E3s as indicated. **, p < 0.001 compared with the first column; Student's t test.
Bafilomycin A1, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PINK1-53 expression is enhanced in the presence of Lys-63-linked ubiquitination. A, anti-PINK1 immunoblots of lysates prepared from control or PINK1-transfected HEK293 cells that were either untreated or treated with 1 μm MG132, 1 μm PSI, 5 μm Lactacytsin, 10 mm 3-methyladenine (3MA), and 200 nm Bafilomycin A1 for 16 h or 10 μm CCCP for 4 h, as indicated. The bands corresponding to full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) are indicated. The blots above were stripped and reprobed with anti-actin as a loading control. This experiment was repeated at least three times. B, representative immunoblots of at least three experimental sets showing the expression level of full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) in the presence of 1 μm MG132 (16 h) or various forms of HA-ubiquitin (Ub). The numbers indicate the average -fold (Avg. fold) change in the densitometric level of PINK1-53. C, same as B, except that HA-ubiquitin was replaced by myc-Ubc13, myc-UbcH7, FLAG-parkin, or HA-HHARI. The asterisk denotes cells treated with 1 μm MG132. This experiment was duplicated. D, representative immunoblots of at least three experimental sets showing the expression level of full-length (63 kDa) and PINK1-53 (53 kDa) in the presence of Ubc13 coexpression alone or with GFP-Ataxin-3, HA-UCHL-1, HA-SENP8, or GFP. (DUB, Deubiquitinating enzyme). E, same as C, except that PINK1 is expressed in some cases in the presence of both myc-Ubc13 and FLAG-parkin or HA-HHARI. The blots above were stripped and reprobed with anti-actin antibody to reflect loading variations. This experiment was duplicated. F, the relative chymotrypsin-like proteasome activities of at least three sets of lysates prepared from PINK1 overexpressing cells cotransfected with various E2s and E3s as indicated. **, p < 0.001 compared with the first column; Student's t test.

Journal: The Journal of Biological Chemistry

Article Title: Cytosolic PTEN-induced Putative Kinase 1 Is Stabilized by the NF-κB Pathway and Promotes Non-selective Mitophagy *

doi: 10.1074/jbc.M114.622399

Figure Lengend Snippet: PINK1-53 expression is enhanced in the presence of Lys-63-linked ubiquitination. A, anti-PINK1 immunoblots of lysates prepared from control or PINK1-transfected HEK293 cells that were either untreated or treated with 1 μm MG132, 1 μm PSI, 5 μm Lactacytsin, 10 mm 3-methyladenine (3MA), and 200 nm Bafilomycin A1 for 16 h or 10 μm CCCP for 4 h, as indicated. The bands corresponding to full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) are indicated. The blots above were stripped and reprobed with anti-actin as a loading control. This experiment was repeated at least three times. B, representative immunoblots of at least three experimental sets showing the expression level of full-length PINK1 (63 kDa) and PINK1-53 (53 kDa) in the presence of 1 μm MG132 (16 h) or various forms of HA-ubiquitin (Ub). The numbers indicate the average -fold (Avg. fold) change in the densitometric level of PINK1-53. C, same as B, except that HA-ubiquitin was replaced by myc-Ubc13, myc-UbcH7, FLAG-parkin, or HA-HHARI. The asterisk denotes cells treated with 1 μm MG132. This experiment was duplicated. D, representative immunoblots of at least three experimental sets showing the expression level of full-length (63 kDa) and PINK1-53 (53 kDa) in the presence of Ubc13 coexpression alone or with GFP-Ataxin-3, HA-UCHL-1, HA-SENP8, or GFP. (DUB, Deubiquitinating enzyme). E, same as C, except that PINK1 is expressed in some cases in the presence of both myc-Ubc13 and FLAG-parkin or HA-HHARI. The blots above were stripped and reprobed with anti-actin antibody to reflect loading variations. This experiment was duplicated. F, the relative chymotrypsin-like proteasome activities of at least three sets of lysates prepared from PINK1 overexpressing cells cotransfected with various E2s and E3s as indicated. **, p < 0.001 compared with the first column; Student's t test.

Article Snippet: Chemicals/reagents used were as follows: MitoTracker Red CMXROS (Molecular Probes); 3-methyladenine, dimethyl sulfoxide (DMSO), 5 TNFα, phorbol 12-myristate 13-acetate (PMA), carbonyl cyanide m -chlorophenylhydrazone (CCCP), and valinomycin (Sigma); clasto-lactacystin-β-lactone (Enzo Life Science), MG132 (A. G. Scientific), Z-IIe-Glu (OtBu)-Ala-Leu-H (aldehyde) (PSI) (Peptides International); Bafilomycin A1 (A. G. Scientific); puromycin (Clontech); BMS-34551 (Sigma); and SC-514 (Calbiochem).

Techniques: Expressing, Ubiquitin Proteomics, Western Blot, Control, Transfection